LOCUS       pLV-[hsa-miR-1183]        9143 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7064..7924
                     /label=Amp(R)
     promoter        8125..8665
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5669..5903
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6478..6933)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5975..6105
                     /label=SV40 pA
     misc_feature    6106..6319
                     /label=SV40 ori
     misc_feature    4990..5586
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3608
                     /label=intron
     CDS             3655..4980
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3398
                     /label=hsa-mir-1183 precursor
BASE COUNT     2196 a      2339 c      2491 g      2117 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcct aagaaggatt 
     3121 aaagactaaa atctgtaaga tgaatagact tttgtcagac caagggagat ggaggtttgg 
     3181 ggtgggagaa tgtgttggat tccagctata ttattcaaat gctcggagac acagaacatt 
     3241 agagaagaca ggagttcact gtaggtgatg gtgagagtgg gcatggagca ggagtgccaa 
     3301 gagatgaagt tataaaacta agcaggagtt agatcatgaa gaactttatg tactatgcta 
     3361 agaaatactg accttattgt gaatgtatgg agggcccagc tagctcgagc ttttggagta 
     3421 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg 
     3481 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt 
     3541 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc 
     3601 catttcaggt gtcgtgaaaa ctacccctct agagtcgagc taccggtcgc caccatggtg 
     3661 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac 
     3721 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc 
     3781 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc 
     3841 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc 
     3901 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg 
     3961 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag 
     4021 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag 
     4081 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg 
     4141 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc 
     4201 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc 
     4261 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc 
     4321 gagggccgcc actccaccgg cggcatggac gagctgtaca aggacccacc ggtcgccacc 
     4381 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 
     4441 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 
     4501 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 
     4561 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 
     4621 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 
     4681 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 
     4741 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 
     4801 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 
     4861 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 
     4921 gacgtcgagt gcccgaagga ccgcgcgacc tggtgcatga cccgcaagcc cggtgcctga 
     4981 gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 
     5041 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 
     5101 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 
     5161 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 
     5221 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 
     5281 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 
     5341 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct 
     5401 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 
     5461 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 
     5521 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 
     5581 cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct 
     5641 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca 
     5701 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct 
     5761 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca 
     5821 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta 
     5881 gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata 
     5941 acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg 
     6001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc 
     6061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg 
     6121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 
     6181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 
     6241 cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg 
     6301 agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg 
     6361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg 
     6421 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg 
     6481 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta 
     6541 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt 
     6601 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg 
     6661 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat 
     6721 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac 
     6781 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag 
     6841 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg 
     6901 cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg 
     6961 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     7021 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     7081 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     7141 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     7201 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
     7261 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
     7321 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     7381 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     7441 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     7501 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     7561 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     7621 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     7681 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     7741 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     7801 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     7861 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     7921 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
     7981 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     8041 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     8101 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     8161 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     8221 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa 
     8281 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     8341 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     8401 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     8461 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     8521 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     8581 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     8641 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     8701 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     8761 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
     8821 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa 
     8881 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 
     8941 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 
     9001 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 
     9061 atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta 
     9121 aagggaacaa aagctggagc tgc 
//
